Expression of Lily mottle virus Coat Protein and Preparation of IgY Antibody against the Recombinant Coat Protein
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چکیده
منابع مشابه
preparation of polyclonal antibody against recombinant coat protein of cucumber mosaic virus isolate b13
cucumber mosaic virus (cmv) is one of widely-spread viruses of plants with the broadest host range encompassing over 1200 species. one major limiting factor for detection of the virus is unavailability of the virus-specific antibodies especially in developing countries. recombinant dna technology facilitates antibody preparation without requiring special equipment. in this study, coat protein (...
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Background: The advent of recombinant DNA technology has facilitated heterologous expression of proteins from various sources in different host systems including Escherichia coli. If a plant virus coat protein is expressed in the bacterium it can be used as the antigen for antibody preparation. Such a recombinant antigen preparation can be particularly useful where equipment such as ultracentri...
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there are many studies related to the production of a elisa kit for diagnosing virus infections. however, production of most kits depends on purification of whole virus particles, which involves the use of costly equipment and reagents. the purpose of this study was to check out if the anti-cp42 antibodies could be used as a diagnostic assay for detection of grapevine fanleaf virus (gflv). in t...
متن کاملPrimary structure of belladonna mottle virus coat protein.
The coat protein of belladonna mottle virus (a tymovirus) was cleaved by trypsin and chymotrypsin, and the peptides were separated by high performance liquid chromatography using a combination of gel permeation, reverse phase, and ion pair chromatography. The peptides were sequenced manually using the 4-N, N-dimethylaminoazobenzene-4'-isothiocyanate/phenyl isothiocyanate double-coupling method....
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Background and Amis: Serological assay is considered as one of the best choices for conducting large number of infection tests. Recombinant DNA technology has been used for expression of virus coat protein (CP) gene in prokaryotic bacterial cells such as Escherichia coli and the recombinant CP (rCP) is used as immunogen in antibody production. Heterologous CP protein expression and purification...
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ژورنال
عنوان ژورنال: Korean Journal of Horticultural Science and Technology
سال: 2014
ISSN: 1226-8763
DOI: 10.7235/hort.2014.13167